4Pi-BRAINSPOT

Nature Communications 2026

Interferometric
ultra-high resolution
3D imaging through
brain sections

4Pi-BRAINSPOT integrates 4Pi single-molecule switching nanoscopy, dynamic in-situ coherent PSF retrieval, tissue clearing, light-sheet illumination, and molecular-coordinate analysis for nanoscale 3D imaging in thick mouse brain sections.

Read article GitHub repository Download toolbox
Authors

Hao-Cheng Gao, Fan Xu, Xi Cheng, Tailong Chen, Cheng Bi, Yue Zheng, Yilun Li, Yumian Li, Alexander A. Chubykin, Fang Huang

Affiliations

Purdue University ยท Beijing Institute of Technology

sub-15 nm3D molecular resolution
50 µmmouse brain slices
6.4 / 2.9 nmlateral / axial precision
4Pi-INSPRin-situ coherent PSF retrieval

Supplementary videos

4Pi-BRAINSPOT media

Supplementary video 1
Supplementary video 2
Supplementary video 3
Supplementary video 4